Recombinant DNA Lab

Our antibiotic resistance was to ampicillan and we found the restriction enzyme Asp II once in the plasmid and twice in the human cell gene, above and below the insulin gene. The bacteria fit in with the other DNA and made a longer strand.
1. I would use ampicillan because the bacteria will resist the antibiotic if the bacteria was taken in. I would not use tetracycline or kanamycine because the plasmid does not have resistance to either antibiotic so those antibiotics would not show if the bacteria is accepted or not.
2. Restriction enzymes are enzymes that are used to recombine DNA by finding a specific nucleotide sequence and cutting it out making a sticky end. We used Asp II because we found it on the plasmid once and the strand twice.
3.  If the plasmid was cute twice then the plasmid would have too many sticky ends and the two places on the end of the plasmid don't fit in with the DNA strand. Sticky ends can only connect if both ends are from the same restriction enzyme.
4. This process is used in the genetically altered food that we eat and buy from grocery stores. This kind of technology changes the way plants grow in different seasons for farmers and consumers.
5. Recombinant DNA is used in insect-resistant and herbicide-resistant plants for farming and gardening. It is also used in Hepatitis B vaccine and the diagnosis of HIV which is very important in the medical world for everyone.