Friday, January 22, 2016

pGLO Lab

1.
Plate
Number of Colonies
Color of colonies under room light
Color of colonies under   UV light
- pGLO LB
carpet
beige
beige
- pGLO LB/amp
0
none
none
+ pGLO LB/amp
4
beige
beige
+ pGLO LB/amp/ara
24
beige
green
2. The new transformed bacteria has resistance to antibiotics and has the trait for glowing under UV light.

3. I estimate around 20-30 bacterium were in the 100 mircoliters because the +pGLO LB/AMP has only 4 colonies that were started but +pGLO LB/AMP/ARA ended with 24 colonies.

4. Arabinose turns on the pGLO gene to glow under UV light.

5. GFP can be used to identify certain bacteria to see if it is present or not in a certain solution, it can be used to make animals glow in the dark maybe as an adaptation, and it can show if an inserted gene has been taken in by glowing in the making of GMOs.

6. Genetic engineering has also been used for making pesticide and herbicide resistant plants for farmers to have a greater crop and produce the most food possible.

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